You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
1 & 2. Acid-fast stain showing both positive (Mycobacteria) and negative (Staphylococcus) results.
Non-acid Fast Bacteria Photos (Click on image to enlarge.)
Mixed +/- Acid Fast Stain Photos (Click on image to enlarge.)
Acid Fast Bacteria Photos (Click on image to enlarge.)
1. First step of acid-fast staining procedure. Applying carbolfuchsin stain to bacterial smear and heating over water bath for 3 - 5 minutes; 2. Rinsing slide after application of crystal violet counterstain; 3. Acid-fast stained slide with + control (Mycobacterium) on left, negative contol (nonacid fast bacteria) on right and unknown in center.
Acid-fast Staining Procedure Photos (Click on image to enlarge.)
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How to Do an Acid-fast
Acid-fast Bacterial Smear and Staining Videos
Page last updated: 8/2013
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