Science Image Library: Acid-fast Stain

Science Image Library
Acid Fast Stain

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Acid-fast stain showing both positive (Mycobacteria) and negative (Staphylococcus) @1000xTM

1. Acid-fast stain showing negative (E. coli) results @ 1000xTM
2. Acid-fast stain showing negative (Staphylococcus) results @ 1000xTM
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1 & 2: Acid-fast stain showing positive (Mycobacteria) results @ 1000xTM.
1 & 2. Acid-fast stain showing both positive (Mycobacteria) and negative (Staphylococcus) results.

Non-acid Fast Bacteria Photos (Click on image to enlarge.)
Mixed +/- Acid Fast Stain Photos (Click on image to enlarge.)
Acid Fast Bacteria Photos (Click on image to enlarge.)
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SCIENCE PHOTOS
1. First step of acid-fast staining procedure. Applying carbolfuchsin stain to bacterial smear and heating over water bath for 3 - 5 minutes; 2. Rinsing slide after application of crystal violet counterstain; 3. Acid-fast stained slide with + control (Mycobacterium) on left, negative contol (nonacid fast bacteria) on right and unknown in center.
Acid-fast Staining Procedure Photos (Click on image to enlarge.)
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How to Prepare a Bacterial Smear for 
Acid Fast (Ziehl-Neelsen) Stain
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How to Do an Acid-fast 
Ziehl-Neelsen Stain

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Page last updated: 5/2014
Instructor's Corner
Applying Carbol Fuchsin to Bacterial Smear Over Water Bath
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The first step of the acid-fast stain, application of carbolfuchsin, is done over a heated water bath.
Acid-fast bacteria Mycobacterium smegmatis viewed under oil immersion @ 1000xTM
Acid-fast bacteria Mycobacterium smegmatis viewed under oil immersion @ 1000xTM



Photographic guides to differential stains 
now available!
1. Gram
2. Acid-fast
3. Endopsore