Transferring Bacteria Using an Inoculation Loop
After the inoculation wand is sterilized and given a moment to cool, the wire end with the loop is gently touched to a bacterial colony on a source plate, or dipped into a test tube of liquid inoculate, and then that sample is smeared onto a new sterile plate, typically using streak plate technique.
Inoculation Loop to Transfer Bacteria - P2
There are typically two ways to sterilize an inoculation wand in a microbiology laboratory--by using an open flame, such as a Bunsen burner, or, a safer and more convenient way, to use a microincinerator.
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Red hot hub of a microincinerator
The freshly inoculated plate is then incubated for at least 24 hours, after which time many types of bacterial colonies should be visible.
Sources & Resources
- Bauman, R. (2014) Microbiology with Diseases by Taxonomy, 4th ed., Pearson Benjamin Cummings.
- Nester, E. et al (2001) Microbiology: A Human Perspective. McGraw Hill.
- Schauer Cynthia (2007) Lab Manual to Microbiology for the Health Sciences, Kalamazoo Valley Community College.
- Tortora, G., Funke, B., Case, C. (2010) Microbiology, an Introduction, Benjamin Cummings.