Transferring Bacteria Using an Inoculation Loop
After the inoculation wand is sterilized and given a moment to cool, the wire end with the loop is gently touched to a bacterial colony on a source plate, or dipped into a test tube of liquid inoculate, and then that sample is smeared onto a new sterile plate, typically using streak plate technique.

Inoculation Loop to Transfer Bacteria - P2
Opening of heated microincinerator​
There are typically two ways to sterilize an inoculation wand in a microbiology laboratory--by using an open flame, such as a Bunsen burner, or, a safer and more convenient way, to use a microincinerator.

Inoculation Loop Used ro Apply a Bacterial  Sample to TSY Agar
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Prokaryotic Cell, Mariana Ruiz
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Red hot hub of a microincinerator
The freshly inoculated plate is then incubated for at least 24 hours, after which time many types of bacterial colonies should be visible.

Sources & Resources
  • Bauman, R. (2014) Microbiology with Diseases by Taxonomy, 4th ed., Pearson Benjamin Cummings.
  • Nester, E. et al (2001) Microbiology: A Human Perspective. McGraw Hill.
  • Schauer Cynthia (2007) Lab Manual to Microbiology for the Health Sciences, Kalamazoo Valley Community College.
  • Tortora, G., Funke, B., Case, C. (2010) Microbiology, an Introduction, Benjamin Cummings.
Micrococcus luteus