Endospore Stain Bacteria Images

Science Image Library
Endospore Stain

from Science Prof Online

Endospore stained Bacillus subtilis @ 1000xTM

All five photos show endospore stain of endospore producing bacteria. Endospores stain green and vegetative, living cells stain red / pink.
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Prokaryotic Cell, Mariana Ruiz
Endospore Stain Positive Result Photos (Click on image to enlarge.)
Endospore Stain Negative Result Photos (Click on image to enlarge.)
Endospore stain of non-endospore producing bacteria. Note lack of green endospores.
Endospore Staining Procedure Photos (Click on image to enlarge.)
1, 2 & 3. Malachite green staining step of endospore stain procedure. Steam from heated water bath supplies heat to drive green stain into endospores; 4 & 5. Safranin couterstain of endospore staining procedure; 6. Endospore stained slide with + control (Bacillus) on left, negative control (E. coli) on right and unknown in center.
Page last updated 10/2014
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How to Do an 
Endospore Bacterial Stain
How to Prepare a Bacterial Smear for Endospore Staining
Endospore Bacterial Smear & Staining Procedure Videos
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Helpful Links on Endospores & Endospore Staining
Gram stained, Acid-fast stained and endospore stained slide (left to right), each with bacterial controls (knowns) on outside and unknown bacterial smear in center.
Gram stained, Acid-fast stained and endospore stained slide (left to right), each with bacterial controls (knowns) on outside and unknown bacterial smear in center.
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