Science Image Library: Bacterial Stains


Science Image Library
Bacterial Staining (Gram, Acid-fast, Endospore)

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Safranin Step of Gram Stain

1. Acid-fast stain showing AF (hot pink) and NAF (purple) bacteria @1000xTM; 2. Acid-fast stained bacterial smears, with acid-fast control (Mycobacterium) on left, nonacid-fast control on right, and unknown in center; 3. Acid fast stained Mycobacteria smegmatis @ 1000xTM; 4. Applying acid-fast primary stain, carbolfuchsin over water bath; 5. Nonacid-fast bacteria Staphylococcus.

1. Gram-positive stained Staphylococcus @100xTM.
2. Gram negative stained E. coli
3. Crystal violet stain step of Gram staining procedure
4.  Safrinin stain step of Gram staining procedure
5. Gram-stained slide with controls on left and right, and unknown in center.

1. Endospore stain of Bacillus subtilis showing both endospores (green) & vegetative cells (pink)  @1000xTM; 2. Negative endospore stain showing only vegetative cells @1000xTM; 3. Malachite green primary staining step of endopore stain with slide being heated over water bath; 4. Applying counterstain (safrinin) to bacterial smear as last step of endospore stain; Endospore stained slide, with + control Bacillus on left, negative control E. coli on right, and unkown in center.

Acid-fast Stain Photos (Click on photo to enlarge. See more)
Endospore Stain Photos (Click on photo to enlarge. See more)
Gram Stain Photos (Click on photo to enlarge. See more)
Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.
Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.
Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.
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Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.
Simple Stain Photos (Click on photo to enlarge. See more)
1. Bacillus subtilis stained with crystal violet @ 100xTM. Cannot see individual bacteria at this magnification.
2. Bacillus subtilis stained with crystal violet @ 1000x TM.
3. E. coli stained with crystal violet @ 100x TM. Cannot see individual bacteria at this magification.
4. E coli stained with crystal violet @ 1000xTM.
5. Cheek cell and oral bacteria stained with crystal violet @ 1000xTM.
Page last updated: 5/2014

Microbiology Photo Libarary
SCIENCE PHOTOS



Photographic guides to differential stains 
now available!
1. Gram
2. Acid-fast
3. Endopsore

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Differential Bacterial Stain Videos
How to Do a Gram Stain
How to Do an Acid-fast 
(Ziehl-Neelsen)  Bacterial Stain
How to Prepare a Bacterial Smear for Gram Staining
How to Prepare a Bacterial Smear for Acid-fast (Ziehl-Neelsen) Staining
How to Do an 
Endospore Bacterial Stain
How to Prepare a Bacterial Smear for Endospore Staining
Microbiology Lab Videos
SCIENCE VIDEOS
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Sources and Resources
  • Schauer, Cynthia (2007) Lab Manual to Microbiology for the Health Sciences, Kalamazoo Valley Community College.
  • Bauman, R. (2014) Microbiology with Diseases by Taxonomy 4th ed., Pearson Benjamin Cummings.
  • Gram Stain Bite Sized Tutorial: This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells.

Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.
Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.