Bacterial Cell Wall & Differential Staining Review Questions

Bacterial Cell Wall & Differential Staining
 Review Questions

Virtual Microbiology Classroom of Science Prof Online


Bacterial Cell Wall Review Questions
Bacterial ​Cell Wall Practice Test Questions

​These multiple choice and true/false questions are designed to help students practice and test their understanding of this topic.

Gram Stained Bacterial Smear
1. Draw a detailed, labeled diagram of Gram-positive  and Gram-negative cell wall structure.

2. Going through each step of the Gram stain, describe why Gram positive bacteria stain purple and Gram negative bacterial stain pink.

​​3. The Gram-negative cell wall contain an endotoxin. Which part of the Gram-negative bacterial cell wall is an endotoxin, and what type of immune response does this toxin trigger?

4. Draw a detailed, labeled diagram of an acid-fast cell wall.

5. Going through each step of the acid-fast stain, describe why acid-fast bacteria, such as Mycobacteria, stain pink and nonacid-fast bacteria stain purple.

6. Which two genera of bacteria are acid-fast, and how does the characteristic of being acid fast benefit these bacteria? What negative effect does being acid-fast have on these bacteria?

7. In differential staining, the bacteria that retain the primary stain are positive for that test. Describe how this rule applies to the Gram stain and acid-fast stain.

Page last updated: 2/2016
These are review questions from the Virtual Microbiology Classroom, designed to help students better understand the history of microbiology. They are based on materials that can be found on the Bacterial Cell Wall & Differential Staining Main Page.

Virtual Microbiology

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Prokaryotic Cell, Mariana Ruiz
Gram stained bacterial smear.